Example Applications for ClonoMapTM in TCR sequencing:
  • Predict and monitor response to immune checkpoint inhibitors.
  • Evaluate immune-related adverse events (IRAE) in immunotherapy regimens.
  • Profile tumor infiltrating lymphocytes.


The T-cell receptor (TCR) repertoire plays a vital role in the immune system and its diversity directly measures immune competence. TCR diversity results from VDJ gene recombination, random insertion, deletion and substitution which generates between 1015 and 1020 clonotypes. With the advent of high-throughput sequencing technology it is now possible to sequence a large number of cells for these genes. Successful high-throughput sequencing and T-cell receptor repertoire analysis requires accurate identification of rare clonal variants and true homologous variants generated from PCR from sequencing errors. Here, we present ClonoMapTM, a kit and analysis engine combining 5’ Rapid Amplification of cDNA Ends (RACE) and the incorporation of molecular barcodes to allow full-length profiling, improved quantification of clonal variants, and error-free analysis of receptor repertoire diversity. Our cloud based web software analyzes raw data, annotates VDJ regions, and clusters clonotypes. Our analysis engine also dynamically visualizes repertoire diversity and testing across different sequencing samples. ClonoMapTM is a robust and reliable research tool that can monitor T cell repertoire changes in cancer immunotherapy, autoimmune diseases, and infectious diseases.
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MIODx pipeline for immune repertoire profiling

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5’RACE based library preparation kit

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•Incorporation of unique molecular barcodes for PCR bias correction

•Full-length V(D)J library generation

•Comprehensive and unbiased TCR deep sequencing provide a representative and quantitative repertoire analysis of specific cellular immunity

 

ClonoMap – A cloud based data analysis engine

ClonoMap™ is a cloud-based web analysis platform designed to manage information about biological samples, sequencing experiments and statistical analysis related to the analysis of immune repertoires.

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Data workflow

From the user point of view, the interaction is entirely made through the web clonomap.com, and a typical workflow begins with the input of metadata from the samples, continues with the analysis of the sequences and finishes with the inspection of the results through data visualizations.

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Data visualization tools 

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References

  • Mahe E, Pugh T, Kamel-Reid S T cell clonality assessment: past, present and future Journal of Clinical Pathology Published Online First: 21 October 2017. doi: 10.1136/jclinpath-2017-204761